探讨人乳头瘤病毒(human papillomavirus，HPV)L1衣壳蛋白在新疆哈萨克族食管病变中的表达差异性及临床意义。

收集2015年1月至2016年12月在新疆维吾尔自治区人民医院经胃镜检查获得的100例哈萨克族食管病变标本，采用HC2二代杂交捕获法检测HPV DNA，其中56例为阳性，阳性标本采用CytoReact HPV L1试剂盒检测壳蛋白在食管脱落细胞中的表达，并追踪其相对应的病理结果，依据病理学结果进行分组，其中食管炎组32例，低级别上皮内瘤变组3例，高级别上皮内瘤变组6例，食管鳞状细胞癌组15例。

56例患者HPVL1壳蛋白的总阳性率为42.8%(24/56)，其中食管炎、低级别上皮内瘤变，高级别上皮内瘤变和食管鳞状细胞癌HPVL1壳蛋白的阳性表达率分别为62.5%(20/32)、66.7%(2/3)、33.3%(2/6)、0(0/15)。炎症组及低级别上皮内瘤变组HPV-L1衣壳蛋白阳性率明显升高，高级别上皮内瘤变组及食管鳞状细胞癌组HPV-L1衣壳蛋白阳性率明显下降。食管鳞状上皮癌组与炎症组、低级别上皮内瘤变组及高级别上皮内瘤变组阳性率比较差异有统计学意义(P值分别为0.000 05、0.000 7、0.000 09)，炎症组与低级别上皮内瘤变组二者间阳性率差异无统计学意义(P>0.05)，低级别上皮内瘤变组与高级别上皮内瘤变组阳性率比较差异无统计学意义(P>0.05)。

HPVL1壳蛋白在新疆哈萨克族食管病变中的阳性表达随病变程度的加重而呈下降趋势，表达存在差异性，提示其有望成为预测新疆哈萨克族食管癌前病变的指标之一。

To discuss the differential expression of human papillomavirus(HPV)-L1 capsid protein and its clinical significance in Kazakh esophageal lesions in Xinjiang.

From January 2015 to December 2016, 100 cases of Kazak esophageal lesions were obtained by endoscopy in Xinjiang Uygur Autonomous Region People′s Hospital.The HPV DNA was detected by hybridcapture 2(HC2)method.The 56 cases were positive.Positive samples were detected by CytoReact HPV-L1 kit to detect the expression of the capsid protein in esophageal exfoliated cell, and the corresponding pathological results were traced.According to the pathological results, 56 cases were divided into esophagitis group(n=32), low grade intraepithelial neoplasia group(n=3), high grade intraepithelial neoplasia group(n=6)and esophageal squamous cell carcinoma group(n=15).

The total positive rate of HPV-L1 capsid protein in 56 cases was 42.8%(24/56). The positive rates of HPV-L1 capsid protein in esophagitis, low grade intraepithelial neoplasia, high grade intraepithelial neoplasia and esophageal squamous cell carcinoma groups were 62.5%(20/32), 66.7%(2/3), 33.3%(2/6), 0(0/15)respectively.The positive rates of HPV-L1 capsid protein were significantly increased in esophagitis group and low grade intraepithelial neoplasia group.The positive rates of HPV-L1 capsid protein were significantly decreased in highgrade intraepithelial neoplasia group and esophageal squamous cell carcinoma group.The positive rates were significantly different between esophageal squamous cell carcinoma group and esophagitis group, low grade intraepithelial neoplasia group and high grade intraepithelial neoplasia group(P=0.000 05; P=0.000 7; P=0.000 09). There was no significant difference in the positive rate between esophagitis group and low grade intraepithelial neoplasia group(P>0.05). The positive rates of low grade intraepithelial neoplasia group and high grade intraepithelial neoplasia group were no significantly different(P>0.05).

The positive expression of HPV-L1 capsid protein in the esophageal lesions in Xinjiang Kazak decreases gradually with the severity of the disease.There are differences in the expression of this protein.It is expected to be one of the indexes to predict the esophageal precancerous lesion in Xinjiang Kazak.