整合素β3表达与结直肠癌淋巴结转移的相关性

doi:10.3877/cma.j.issn.2095-2015.2020.03.002

中华消化病与影像杂志(电子版) ›› 2020, Vol. 10 ›› Issue (03) : 101 -104. doi: 10.3877/cma.j.issn.2095-2015.2020.03.002

所属专题：文献；

临床研究

整合素β3表达与结直肠癌淋巴结转移的相关性

李红磊¹, 柴小雪¹, 朱婷婷¹, 赵传喜¹, 刘明国², 曹莉莉¹^,^*(

)

^1. 250014　济南，山东大学附属千佛山医院风湿免疫病转化医学重点实验室
^2. 274700　山东菏泽，菏泽郓城诚信医院内科

收稿日期:2020-04-27 出版日期:2020-06-01
通信作者: 曹莉莉
基金资助:
山东省重点研发计划(2019GSF108180); 山东省中医药科技发展计划(2019-0378); 济南市科技发展计划(201907119); 山东第一医科大学第一附属医院培育基金(QYPY2019NSFC1015)

Correlation between expression of integrin β3 and lymph node metastasis in colorectal cancer

Honglei Li¹, Xiaoxue Chai¹, Tingting Zhu¹, Chuanxi Zhao¹, Mingguo Liu², Lili Cao¹^,^†()

^1. Key Laboratory of Rheumatoid Immunology Translation Medicine, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China
^2. Department of Internal Medicine, Heze Yuncheng Chengxin Hospital, Heze 274700, China

Received:2020-04-27 Published:2020-06-01
Corresponding author: Lili Cao
About author:
Corresponding author: Cao Lili, Email: cll@sdu.edu.cn

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引用本文：

李红磊, 柴小雪, 朱婷婷, 赵传喜, 刘明国, 曹莉莉. 整合素β3表达与结直肠癌淋巴结转移的相关性[J/OL]. 中华消化病与影像杂志(电子版), 2020, 10(03): 101-104.

Honglei Li, Xiaoxue Chai, Tingting Zhu, Chuanxi Zhao, Mingguo Liu, Lili Cao. Correlation between expression of integrin β3 and lymph node metastasis in colorectal cancer[J/OL]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2020, 10(03): 101-104.

目的

探讨结直肠癌患者肺转移重要的血管内皮细胞标志物整合素β3（ITGB3）表达与结直肠癌转移之间的相关性。

方法

采用免疫组织化学染色法检测49例原发性结直肠癌患者癌组织、癌旁正常肠黏膜组织以及相应淋巴结组织中ITGB3的表达。其中37例患者有淋巴结转移。分析ITGB3表达与患者结直肠癌转移的相关性。

结果

免疫组化染色结果显示，ITGB3主要在原发性结直肠癌组织、癌旁正常肠黏膜细胞质以及相应淋巴结的间质中表达。ITGB3在不同组织的表达不同，在癌组织中的表达低于癌旁正常肠黏膜组织，且差异有统计学意义（P<0.01）；有淋巴结转移患者淋巴结ITGB3表达高于无淋巴结转移患者，且差异有统计学意义（P<0.001）。淋巴结上皮细胞ITGB3的表达与淋巴结间质组织的表达呈正相关（r=0.395，P=0.005）；且有淋巴结转移患者癌组织上皮细胞ITGB3表达与淋巴结上皮细胞ITGB3表达呈正相关（r=0.514，P=0.001）。ITGB3在淋巴结表达、上皮细胞表达以及间质表达均与淋巴结转移呈正相关（r=0.659，P<0.0001；r=0.661，P<0.0001；r=0.354，P=0.013）。

结论

ITGB3淋巴结表达与结直肠癌淋巴结转移呈正相关。ITGB3可能是原发性结直肠癌患者淋巴结转移的潜在分子标志物。

关键词: 原发性结直肠癌, 整合素β3, 淋巴结转移

Objective

To investigate the correlation between the expression of integrin β3 (ITGB3, an important vascular endothelial cell marker) and lymph node metastasis in colorectal cancer patients.

Methods

Immunohistochemical (IHC) staining was used to detect the expression of ITGB3 in cancer tissues, normal mucosa tissues adjacent to cancer and corresponding lymph node tissues of 49 patients with primary colorectal cancer. Thirty-seven patients had lymph node metastasis. The correlation between ITGB3 expression and cancer metastasis was analyzed.

Results

IHC results showed that ITGB3 positive immunoreaction was mainly observed in the cytoplasm of primary colorectal cancer, normal mucosa adjacent to cancer and mesenchyme of corresponding lymph nodes. ITGB3 had different expression in different tissues. The expression of ITGB3 in cancer tissues was significantly lower than that in normal tissues adjacent to cancer, with a statistically significant difference (P<0.01). The expression of ITGB3 in lymph nodes of metastatic group was significantly higher than that of non-metastatic group, with a statistically significant difference (P<0.001). There was a positive correlation between the expression of ITGB3 in lymph node epithelial cells and the expression in lymph node interstitial tissue (r=0.395, P=0.005). In lymph node metastatic group, there was a positive correlation between the expression of ITGB3 in epithelial cells of cancer tissue and the expression in lymph node epithelial cells (r=0.514, P=0.001). The expressions of ITGB3 in lymph nodes, epithelial cells and mesenchyme were positively correlated with lymph node metastasis.

Conclusions

The expression of ITGB3 in lymph nodes are positively correlated with colorectal cancer lymph node metastasis. ITGB3 may be a potential molecular marker for lymph node metastasis in patients with primary colorectal cancer.

Key words: Primary colorectal cancer, Integrin β3, Lymph node metastasis

图1 整合素β3的亚细胞定位（免疫组织化学染色法）。有淋巴结转移患者中，ITGB3的阳性反应（染为棕色）主要出现在结直肠癌、癌旁正常黏膜组织上皮细胞的细胞质、相应淋巴结的间质中；无淋巴结转移患者中，结直肠癌、癌旁正常黏膜组织的细胞质中存在ITGB3的阳性反应（染为棕色），而淋巴结中并未观察到阳性染色。图A、B、C为有淋巴结转移患者，图D、E、F为无淋巴转移患者；图A、D为结直肠癌组织，图B、E为癌旁正常黏膜组织，图C、F为淋巴结；标尺为100 μm

表1 整合素β3在结直肠癌不同组织的表达（±s）

组织	例数	有淋巴结转移(n=37)		无淋巴结转移(n=12)		合计
组织	例数	上皮细胞	间质	上皮细胞	间质	合计
结直肠癌组织	49	0.236±0.051	0.964±0.070	0.379±0.070	0.490±0.083	0.876±0.071
癌旁正常黏膜组织	49	0.156±0.038	0.639±0.063	0.140±0.063	1.092±0.062	1.147±0.064
P值	?	0.2142	0.0009	0.0181	<0.001	0.0049

表1 整合素β3在结直肠癌不同组织的表达（±s）

组织	例数	有淋巴结转移(n=37)		无淋巴结转移(n=12)		合计
组织	例数	上皮细胞	间质	上皮细胞	间质	合计
结直肠癌组织	49	0.236±0.051	0.964±0.070	0.379±0.070	0.490±0.083	0.876±0.071
癌旁正常黏膜组织	49	0.156±0.038	0.639±0.063	0.140±0.063	1.092±0.062	1.147±0.064
P值	?	0.2142	0.0009	0.0181	<0.001	0.0049

1	Brody H. Colorectal cancer [J]. Nature, 2015, 521(7551): S1.
2	Thanikachalam K, Khan G. Colorectal cancer and nutrition [J]. Nutrients, 2019, 11(1): 164.
3	Tauriello DV, Calon A, Lonardo E, et al. Determinants of metastatic competency in colorectal cancer [J]. Mol Oncol, 2017, 11(1): 97-119.
4	Rahbari NN, Carr PR, Jansen L, et al. Time of metastasis and outcome in colorectal cancer [J]. Ann Surg, 2019, 269(3): 494-502.
5	Huang D, Sun W, Zhou Y, et al. Mutations of key driver genes in colorectal cancer progression and metastasis [J]. Cancer Metastasis Rev, 2018, 37(1): 173-187.
6	Yu S, Bian H, Gao X, et al. Annexin A9 promotes invasion and metastasis of colorectal cancer and predicts poor prognosis [J]. Int J Mol Med, 2018, 41(4): 2185-2192.
7	Liu J, Liang T, Zhangsun W. KDM3A is associated with tumor metastasis and modulates colorectal cancer cell migration and invasion [J]. Int J Biol Macromol, 2019, 126: 318-325.
8	Coppede F, Lopomo A, Spisni R, et al. Genetic and epigenetic biomarkers for diagnosis, prognosis and treatment of colorectal cancer [J]. World J Gastroenterol, 2014, 20(4): 943-956.
9	De Franceschi N, Hamidi H, Alanko J, et al. Integrin traffic-the update [J]. J Cell Sci, 2015, 128(5): 839-852.
10	Mould AP. Analyzing integrin-dependent adhesion [J]. Curr Protoc Cell Biol, 2019, 82(1): e69.
11	Zhu C, Kong Z, Wang B, et al. ITGB3/CD61: a hub modulator and target in the tumor microenvironment [J]. Am J Transl Res, 2019, 11(12): 7195-7208.
12	Hu L, Zang MD, Wang HX, et al. G9A promotes gastric cancer metastasis by upregulating ITGB3 in a SET domain-independent manner [J]. Cell Death Dis, 2018, 9(3): 278.
13	Wu Q, Zhong H, Jiao L, et al. MiR-124-3p inhibits the migration and invasion of Gastric cancer by targeting ITGB3 [J]. Pathol Res Pract, 2020, 216(1): 152762.
14	Zheng ZQ, Li ZX, Zhou GQ, et al. Long noncoding RNA FAM225A promotes nasopharyngeal carcinoma tumorigenesis and metastasis by acting as ceRNA to sponge miR-590-3p/miR-1275 and upregulate ITGB3 [J]. Cancer Res, 2019, 79(18): 4612-4626.
15	Wei W, Yang Y, Cai J, et al. MiR-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3 [J]. Cell Physiol Biochem, 2016, 39(3): 1165-1176.
16	Stremitzer S, Berghoff AS, Volz NB, et al. Genetic variants associated with colorectal brain metastases susceptibility and survival [J]. Pharmacogenomics J, 2017, 17(1): 29-35.
17	Zhang N, Ma D, Wang L, et al. Insufficient radiofrequency ablation treated hepatocellular carcinoma cells promote metastasis by up-regulation ITGB3 [J]. J Cancer, 2017, 8(18): 3742-3754.
18	Yang MH, Zhao L, Wang L, et al. Nuclear lncRNA HOXD-AS1 suppresses colorectal carcinoma growth and metastasis via inhibiting HOXD3-induced integrin beta3 transcriptional activating and MAPK/AKT signalling [J]. Mol Cancer, 2019, 18(1): 31.

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Correlation between expression of integrin β3 and lymph node metastasis in colorectal cancer

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