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中华消化病与影像杂志(电子版)

中华消化病与影像杂志(电子版) ›› 2022, Vol. 12 ›› Issue (02) : 98 -101. doi: 10.3877/cma.j.issn.2095-2015.2022.02.007

基础研究

人肝窦内皮细胞中α1肾上腺素能受体亚型表达的初步实验研究
丁茜1, 李振1, 王思宁2, 赵连晖2, 王广川1, 张春清3,†()   
  1. 1. 250021　济南，山东第一医科大学附属省立医院消化内科
    2. 250021　济南，山东大学省立医院消化内科
    3. 250021　济南，山东第一医科大学附属省立医院消化内科；250021　济南，山东大学省立医院消化内科
  • 收稿日期:2022-03-04 出版日期:2022-04-01
  • 通信作者: 张春清
  • 基金资助:
    国家自然科学基金(81970533，82000566)

Preliminary experimental study on the expression of α1 adrenergic receptor subtypes in human liver sinusoidal endothelial cells

Qian Ding1, Zhen Li1, Sining Wang2, Lianhui Zhao2, Guangchuan Wang1, Chunqing Zhang3,†()   

  1. 1. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
    2. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
    3. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China; Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2022-03-04 Published:2022-04-01
  • Corresponding author: Chunqing Zhang
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引用本文：

丁茜, 李振, 王思宁, 赵连晖, 王广川, 张春清. 人肝窦内皮细胞中α1肾上腺素能受体亚型表达的初步实验研究[J]. 中华消化病与影像杂志(电子版), 2022, 12(02): 98-101.

Qian Ding, Zhen Li, Sining Wang, Lianhui Zhao, Guangchuan Wang, Chunqing Zhang. Preliminary experimental study on the expression of α1 adrenergic receptor subtypes in human liver sinusoidal endothelial cells[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2022, 12(02): 98-101.

目的

探究α1肾上腺素能受体（α1AR）各亚型在肝窦内皮细胞（LSEC）中的表达情况。

方法

体外培养人原代LSEC，蛋白质印迹法验证α1AR激动剂去氧肾上腺素和α1AR阻滞剂多沙唑嗪对LSEC表达血管生成相关指标CD31和vWF的影响，并验证LSEC中α1AR各亚型的表达情况。免疫组化染色验证小鼠肝脏α1AR各亚型的表达情况。

结果

α1AR激动剂去氧肾上腺素可增加LSEC中CD31和vWF的表达，而α1AR阻滞剂多沙唑嗪可拮抗此作用。体内和体外实验均在LSEC中检测到α1aAR与α1bAR的表达，未检测到α1dAR的表达。

结论

α1AR参与LSEC表型的调控，且通过α1aAR与α1bAR两亚型实现此功能，因此α1AR可能成为肝纤维化新的调控靶点。

关键词: 肝纤维化, α1肾上腺素能受体, 肝窦内皮细胞
Objective

To investigate the expressions of α1 adrenergic receptor (α1AR) subtypes in liver sinusoidal endothelial cells (LSEC).

Methods

Human primary LSEC were cultured in vitro. The effects of α1AR agonists phenylephrine and α1AR blocker doxazosin on the expressions of angiogenesis-related markers CD31 and vWF in LSEC and the expressions of various α1AR subtypes in LSEC were verified by Western blotting analysis. Immunohistochemistry was used to verify the expressions of α1AR subtypes in mouse liver.

Results

α1AR agonist phenylephrine could increase the expressions of CD31 and vWF in LSEC, and α1AR blocker doxazosin antagonized this effect. The expressions of α1aAR and α1bAR were detected in LSEC in vivo and in vitro, but the expression of α1dAR was not detected.

Conclusions

α1AR is involved in the regulation of LSEC phenotype, and this function is achieved through two subtypes of α1aAR and α1bAR. Therefore, α1AR may become a new regulatory target of liver fibrosis.

Key words: Liver fibrosis, α1 adrenergic receptor, Liver sinusoidal endothelial cells
图1 蛋白质印迹法检测肝窦内皮细胞表达CD31、vWF。PE为去氧肾上腺素；Dox为多沙唑嗪
表1 CD31蛋白及vWF蛋白表达水平半定量分析（±s）
组别 CD31/Tubulin相对灰度值 vWF/Tubulin相对灰度值
对照组 1 1
去氧肾上腺素组 1.614±0.202a 1.246±0.150a
去氧肾上腺素+多沙唑嗪组 0.969±0.153b 0.761±0.151b
图2 人肝窦内皮细胞α1AR各亚型表达。图A为蛋白质印迹法检测肝窦内皮细胞α1AR 3种亚型α1aAR、α1bAR和α1dAR的表达水平；图B为免疫组织化学染色检测大鼠肝组织中α1AR 3种亚型α1aAR、α1bAR和α1dAR的表达水平（×20）。箭头示肝窦内皮细胞
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