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中华消化病与影像杂志(电子版) ›› 2024, Vol. 14 ›› Issue (06) : 485 -490. doi: 10.3877/cma.j.issn.2095-2015.2024.06.002

论著

miR-34a-5p 在结肠癌患者的表达及其与临床特征及预后的相关性研究
刘郁1, 段绍斌1,(), 丁志翔1, 史志涛1   
  1. 1.830000 乌鲁木齐,新疆医科大学附属中医医院普外一科
  • 收稿日期:2024-03-05 出版日期:2024-12-01
  • 通信作者: 段绍斌
  • 基金资助:
    新疆维吾尔自治区自然科学基金资助(2021D01C235)

Expression of miR-34a-5p in colon cancer patients and its correlation with clinical characteristics and prognosis

Yu Liu1, Shaobin Duan1,(), Zhixiang Ding1, Zhitao Shi1   

  1. 1.Department of General Surgery,Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital,Urumqi 830000,China
  • Received:2024-03-05 Published:2024-12-01
  • Corresponding author: Shaobin Duan
引用本文:

刘郁, 段绍斌, 丁志翔, 史志涛. miR-34a-5p 在结肠癌患者的表达及其与临床特征及预后的相关性研究[J]. 中华消化病与影像杂志(电子版), 2024, 14(06): 485-490.

Yu Liu, Shaobin Duan, Zhixiang Ding, Zhitao Shi. Expression of miR-34a-5p in colon cancer patients and its correlation with clinical characteristics and prognosis[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2024, 14(06): 485-490.

目的

探讨miR-34a-5p 在结肠癌患者的表达水平,并分析其与结肠癌患者临床特征及预后的相关性。

方法

采用前瞻性队列研究的方法,选择2021 年1 月1 日至2021 年12 月31 日在新疆医科大学附属中医医院接受治疗的结肠癌伴腹膜转移患者,计划入样本60 例,收集患者的癌灶组织和癌旁组织标本,实时定量PCR 检测癌灶组织和癌旁组织标本miR-34a-5p 相对表达量,分析miR-34a-5p 相对表达量与结肠癌患者临床特征的相关性,对结肠癌患者进行随访,绘制Kaplan-Meier 曲线并采用COX 回归分析预后的相关因素。

结果

最终纳入结肠癌患者58 例,癌灶组织miR-34a-5p相对表达量明显低于癌旁组织,差异有统计学意义(P<0.001)。以结肠癌患者癌灶组织miR-34a-5p相对表达量平均值分组,结果显示N 分期、分化程度与miR-34a-5p 表达水平相关。绘制Kaplan-Meier生存曲线,结果显示结肠癌miR-34a-5p 低表达组生存率明显低于高表达组,差异有统计学意义(P=0.028)。单因素分析显示,T 分期、N 分期、分化程度、miR-34a-5p 表达水平与结肠癌患者生存期有关,差异有统计学意义(P<0.05)。多因素COX 回归模型结果提示,分化程度、miR-34a-5p表达水平是结肠癌患者预后生存期的独立影响因素。

结论

在结肠癌患者病灶组织中,miR-34a-5p表达水平降低,miR-34a-5p 表达水平与N 分期、分化程度相关。miR-34a-5p 表达水平是预后生存期的独立影响因素。

Objective

To investigate the expression level of miR-34a-5p in patients with colon cancer and analyze its correlation with the clinical characteristics and prognosis of these patients.

Methods

A prospective cohort study was conducted. Patients with colon cancer accompanied by peritoneal metastasis who underwent treatment in Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital from January 1,2021 to December 31,2021 were selected. A sample of 60 patients was planned,and the cancer focus and adjacent tissue samples of the patients were collected.Real-time quantitative PCR was used to detect the relative expression level of miR-34a-5p in the cancer focus and adjacent tissue samples,and the correlation between the relative expression level of miR-34a-5p and the clinical characteristics of colon cancer patients was analyzed. The patients with colon cancer were followed up,Kaplan-Meier curves were drawn,and prognostic factors were analyzed using COX regression.

Results

In the end,58 patients with colon cancer were included,and the relative expression level of miR-34a-5p in cancer focus tissues was significantly lower than that in adjacent tissues,with a statistically significant difference (P<0.001). Grouped by the average relative expression level of miR-34a-5p in cancer focus tissues of colon cancer patients,the results showed that N staging and differentiation degree were related to the expression level of miR-34a-5p. Drawing Kaplan-Meier survival curves,the results showed that the survival rate of the low expression group of miR-34a-5p in colon cancer was significantly lower than that of the high expression group,with a statistically significant difference(P=0.028). Univariate analysis showed that T staging,N staging,differentiation degree,and miR-34a-5p expression level were related to the survival period of colon cancer patients,with statistically significant differences (P<0.05). Multivariate COX regression model results suggested that differentiation degree and miR-34a-5p expression level were independent factors affecting the prognosis and survival period of colon cancer patients.

Conclusion

In the lesion tissues of colon cancer patients,the expression level of miR-34a-5p decreases,and the expression level of miR-34a-5p is related to N staging and differentiation degree. The expression level of miR-34a-5p is an independent factor affecting prognosis and survival period.

图1 研究技术路线图
图2 65 岁男性结肠癌伴腹膜转移患者病理组织学及免疫组化结果 注:2A 病理组织结果(HE 染色,×200);2B 免疫组化MLH1 阳性;2C MSH2 阳性;2D MSH6 阳性;2E PMS2 阳性;2F CDX2 阳性;2G CK20阳性;2H KI67 阳性;2I Villin 阳性。
图3 结肠癌患者癌灶组织和癌旁组织miR-34a-5p 相对表达水平
表1 结肠癌患者miR-34a-5p 表达与临床特征相关性[例(%)]
图4 结肠癌患者不同miR-34a-5p 表达水平Kaplan-Meier 生存曲线分析
表2 结肠癌患者生存期的单因素和多因素COX 回归分析结果
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