目的

探讨轮状病毒肠炎（RE）患儿血清肠型脂肪酸结合蛋白（I-FABP）、高迁移率族蛋白B1（HMGB1）、T 淋巴细胞亚群变化与病情严重程度的相关性。

方法

选择2021 年4 月至2024 年4 月榆林市第一医院接诊的RE 患儿210 例作为研究组，病情严重程度根据患儿脱水程度将其分为轻中度组（n=151）和重度组（n=59），选择180 例体检健康的儿童作为对照组。比较各组血清I-FABP、HMGB1、T 淋巴细胞亚群变化，采用Pearson 相关分析法分析RE 患儿血清I-FABP、HMGB1、T 淋巴细胞亚群变化与病情严重程度的相关性。

结果

研究组I-FABP、HMGB1、CD8⁺水平明显高于对照组，CD3⁺、CD4⁺、CD4⁺/CD8⁺水平均明显低于对照组（P＜0.05）；重度组I-FABP、HMGB1、CD8⁺水平明显高于轻中度组，CD3⁺、CD4⁺、CD4⁺/CD8⁺水平均明显低于轻中度组（P＜0.05）。Pearson 相关分析显示，RE 患儿血清I-FABP、HMGB1、CD8+水平与病情严重程度呈正相关，CD3⁺、CD4⁺、CD4⁺/CD8⁺水平与病情严重程度呈负相关（P＜0.05）。

结论

RE 患儿血清中I-FABP、HMGB1 及T 淋巴细胞亚群水平与病情严重程度密切相关，监测这些指标可为RE 患儿的病情评估和治疗提供参考。

Objective

To investigate the relationship between the changes of serum intestinal fatty acid binding protein (I-FABP), high mobility group protein B1 (HMGB1), T lymphocyte subsets and the severity of disease in children with rotavirus enteritis (RE).

Methods

A total of 210 children with RE admitted to Yulin First Hospital from April 2021 to April 2024 were selected as the study group, according to the severity of dehydration, the children were divided into mild to moderate group (n=151) and severe group (n=59), and 180 healthy children were selected as the control group.The changes of I-FABP,HMGB1 and T lymphocyte subsets in serum of all groups were compared, Pearson correlation analysis was used to analyze the correlation between the changes of serum I-FABP, HMGB1 and T lymphocyte subsets and the severity of RE in children.

Results

The levels of I-FABP, HMGB1 and CD8⁺ in study group were significantly higher than those in control group, while the levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ in study group were significantly lower than those in control group (P<0.05); the levels of I-FABP, HMGB1 and CD8⁺ in severe group were significantly higher than those in mild to moderate group, while the levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ were significantly lower than those in mild to moderate group (P<0.05).The results of Pearson correlation analysis showed that the serum levels of I-FABP, HMGB1 and CD8⁺ were positively correlated with the severity of the disease, while the levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ were negatively correlated with the severity of the disease (P<0.05).

Conclusion

The levels of I-FABP,HMGB1, and T lymphocyte subsets in the serum of children with RE are closely related to the severity of the condition, and monitoring these indicators can provide an important reference for the evaluation and treatment of children with RE.