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中华消化病与影像杂志(电子版) ›› 2021, Vol. 11 ›› Issue (03) : 117 -120. doi: 10.3877/cma.j.issn.2095-2015.2021.03.004

所属专题: 文献

实验研究

α1肾上腺素能受体阻滞剂降低肝硬化门脉高压的实验研究
修爱媛1, 王思宁1, 丁茜2, 王广川2, 张春清2,()   
  1. 1. 250021 济南,山东大学附属省立医院消化内科
    2. 250021 济南,山东大学附属省立医院消化内科;250021 济南,山东第一医科大学附属省立医院消化内科
  • 收稿日期:2021-01-25 出版日期:2021-06-01
  • 通信作者: 张春清
  • 基金资助:
    国家自然科学基金(81970533)

Experimental study of reducing cirrhotic portal hypertension byα1 adrenergic receptor blocker

Aiyuan Xiu1, Sining Wang1, Qian Ding2, Guangchuan Wang2, Chunqing Zhang2,()   

  1. 1. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
    2. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China; Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
  • Received:2021-01-25 Published:2021-06-01
  • Corresponding author: Chunqing Zhang
引用本文:

修爱媛, 王思宁, 丁茜, 王广川, 张春清. α1肾上腺素能受体阻滞剂降低肝硬化门脉高压的实验研究[J/OL]. 中华消化病与影像杂志(电子版), 2021, 11(03): 117-120.

Aiyuan Xiu, Sining Wang, Qian Ding, Guangchuan Wang, Chunqing Zhang. Experimental study of reducing cirrhotic portal hypertension byα1 adrenergic receptor blocker[J/OL]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2021, 11(03): 117-120.

目的

探究α1肾上腺素能受体(α1AR)阻滞剂在降低肝硬化门脉高压中的作用。

方法

采用随机数字表法将50只小鼠分成5组,肝硬化小鼠造模采用四氯化碳(CCl4)造模法,α1AR阻滞剂选用多沙唑嗪。分为5组:橄榄油组;CCl4组;CCl4+多沙唑嗪2.5 mg/(kg·d)组;CCl4+多沙唑嗪5 mg/(kg·d)组;CCl4+多沙唑嗪10 mg/(kg·d)组。干预结束后,进行门静脉压力测定,并进行统计学分析。

结果

α1AR阻滞剂(多沙唑嗪)可降低肝硬化小鼠的死亡率。与橄榄油组相比,其他4组小鼠的门静脉压力均有明显增高(P均<0.05)。与CCl4组相比,CCl4+多沙唑嗪2.5 mg/(kg·d)组及CCl4+多沙唑嗪5 mg/(kg·d)组的小鼠门静脉压力水平均有降低,但差异均无统计学意义。与CCl4组相比,CCl4+多沙唑嗪10 mg/(kg·d)组的小鼠门静脉压力水平明显降低,差异有统计学意义(P<0.05)。

结论

α1AR阻滞剂(多沙唑嗪)可抑制肝硬化小鼠门静脉压力升高,降低肝硬化小鼠的死亡率,可能为肝硬化门脉高压的治疗提供新思路。

Objective

To explore the role of α1 adrenergic receptor(α1AR)blocker in reducing portal hypertension in liver cirrhosis.

Methods

Fifty mice were divided into 5 groups by random number table method.Carbon tetrachloride(CCl4)was used to model liver cirrhosis, and doxazosin was used as α1AR blocker.The mice were grouped into: ①olive oil group, ②CCl4 group, ③CCl4+ doxazosin 2.5 mg/(kg*day)group, ④CCl4+ doxazosin 5 mg/(kg*day)group, ⑤CCl4+ doxazosin 10 mg/(kg*day)group.At the end of the intervention, portal venous pressure was measured and statistically analyzed.

Results

α1AR blocker(doxazosin)could reduce the mortality of mice with liver cirrhosis.Compared with the olive oil group, the portal venous pressures of the mice in the other four groups were significantly higher(all P<0.05). Compared with the CCl4 group, the portal venous pressure levels of mice in the CCl4+ doxazosin 2.5 mg/(kg*day)group and CCl4+ doxazosin 5 mg/(kg*day)group were statistically reduced, but there were no statistically significant differences(both P>0.05). Compared with the CCl4 group, the portal pressure level of mice in the CCl4+ doxazosin 10 mg/(kg*day)group was significantly reduced, and there was a statistically significant difference(P<0.05).

Conclusion

α1AR blocker(doxazosin)can inhibit the increase of portal venous pressure in cirrhotic mice and reduce the mortality of cirrhotic mice, which may provide new ideas for the treatment of portal hypertension in cirrhosis.

图1 五组小鼠门静脉压力检测统计图
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