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中华消化病与影像杂志(电子版) ›› 2022, Vol. 12 ›› Issue (03) : 158 -161. doi: 10.3877/cma.j.issn.2095-2015.2022.03.008

综述

染色体外环状DNA在消化道肿瘤检测中的应用前景
宗晓琳1, 金宏威2,()   
  1. 1. 100044 北京,国家卫生健康委医药卫生科技发展研究中心
    2. 100191 北京大学药学院天然药物及仿生药物国家重点实验室
  • 收稿日期:2022-03-25 出版日期:2022-06-01
  • 通信作者: 金宏威
  • 基金资助:
    国家自然科学基金面上项目(81670637)

Research progress of eccDNA and its application prospect in clinical detection

Xiaolin Zong1, Hongwei Jin2,()   

  1. 1. Development Center for Medical Science & Technology National Health Commission of the People′s Republic of China, Beijing 100044, China
    2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
  • Received:2022-03-25 Published:2022-06-01
  • Corresponding author: Hongwei Jin
引用本文:

宗晓琳, 金宏威. 染色体外环状DNA在消化道肿瘤检测中的应用前景[J]. 中华消化病与影像杂志(电子版), 2022, 12(03): 158-161.

Xiaolin Zong, Hongwei Jin. Research progress of eccDNA and its application prospect in clinical detection[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2022, 12(03): 158-161.

染色体外环状DNA(eccDNA)是一类来源于染色体的环状DNA,因其在肿瘤发生、异质性和耐药性中的重要作用引起广泛关注。线性的循环核酸(cfDNA)已被广泛研究并成为多种疾病无创诊断和研究的新分子标记物,相较于线性染色体外DNA,eccDNA具有基因信息丰富和稳定性高等优势,更适于作为临床诊断分子标记物。随着检测技术的飞速发展,越来越多研究表明eccDNA不仅广泛存在于人体组织器官,而且具有明显的组织特异性和生理病理特异性,近期针对消化道肿瘤中eccDNA的研究显示了eccDNA作为消化道肿瘤临床检测标记物的良好前景。

Extrachromosomal circular DNA (eccDNA) is a kind of circular DNA derived from chromosomes. It has attracted extensive attention because of its important role in tumorigenesis, heterogeneity and drug resistance. Linear circulating free DNA (cfDNA) has been widely studied and has become a new molecular marker for noninvasive diagnosis and research of a variety of diseases. Compared with linear extrachromosomal DNA, eccDNA is more suitable for clinical diagnosis for the advantages of rich in gene information and high stability. With the rapid development of detection technology, increasing studies show that eccDNA not only widely exists in human tissues and organs, but also has obvious tissue specificity and physiological and pathological specificity. Recent studies on eccDNA in gastrointestinal tumors show that eccDNA has a good prospect as a clinical detection marker of gastrointestinal tumors.

图1 电子显微镜下成年小鼠肾脏组织的双链eccDNA和脾脏组织的单链eccDNA(比例尺为100 nm)[1]
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