Effect and significance of IGFBPrP1 on kidney tissues in SD rats and beagles

doi:10.3877/cma.j.issn.2095-2015.2017.01.006

Abstract

Abstract:

Objective

To investigate the effect and significance of insulin-like growth factor binding protein-related protein1(IGFBPrP1)on kidney tissues in SD rats and beagles.

Methods

(1)Healthy male SD rats were randomly divided into A, B and C group.Rats were respectively injected with the same doses of saline, adenovirus carrying enhanced green fluorescent protein(Ad-EGFP)and Ad-IGFBPrP1 via the tail vein.Kidney tissues were obtained for analysis at week 1, 2, 4, 6 and 9.(2)Healthy male beagles were randomly divided into D, E and F group.Beagles in D group were normally fed.Beagles in E group were treated with subcutaneous injection of thioacetamide(TAA). Beagles in F group were simultaneously given Anti-IGFBPrP1 antibody after 5 weeks with the treatment of TAA.Kidney tissues were obtained for analysis at 12 weeks.Histological changes and deposition of collagen in rats and beagles kidney tissues were evaluated by HE and Sirius red staining.Immunohistochemistry was used to analyze the expressions of IGFBPrP1, interleukin-1β(IL-1β), transforming growth factor-β1(TGF-β1)and fibronectin(Fn)in rats kidney tissues and IGFBPrP1 in beagles.

Results

(1)SD rats: Along with the prolongation of the time, the lesion gradually aggravated and the content of collagen fibers gradually increased in C group comparing with A and B group.In C group, the protein level of IGFBPrP1 increased 1 week after transfection and peaked at 2 weeks after the injection, and then gradually decreased.The expression of IL-1βpeaked at 6 weeks, and later downregulated.The level of TGFβ1 increased in a time-dependent manner and peaked at 9 weeks.Fn markedly upregulated at 9 weeks after injection.(2)Beagles: Compared with D group, obvious pathological changes and collagen fibers deposition were observed in E group.In F group, the lesion in kidney tissue ameliorated.IGFBPrP1 protein level was significantly increased in E group and that was downregulated in F group.

Conclusion

The kidney can be effected to one degree or another by IGFBPrP1, which is a non-tissue-specificity pathogenic factor.

Key words: Insulin-like growth factor binding protein-related protein 1, Kidney, Rats, Sprague-Dawley, Beagles

Yu Guan, Lixin Liu, Yu Guo, Tingting Lyu, Xiaohong Guo, Yarong Guo. Effect and significance of IGFBPrP1 on kidney tissues in SD rats and beagles[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2017, 07(01): 26-33.

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Figures/Tables 13

References 13

1	Perez Aguilar RC, HonoréSM, Genta SB, et al.Hepatic fibrogenesis and transforming growth factor/Smad signaling activation in rats chronically exposed to low doses of lead[J]. J Appl Toxicol, 2014, 34(12): 1320-1331.
2	Schnaper HW, Jandeska S, Runyan CE, et al.TGF-beta signal transduction in chronic kidney disease[J]. Front Biosci(Landmark Ed), 2009, 14: 2448-2465.
3	Liu LX, Huang S, Zhang QQ, et al.Insulin-like growth factor binding protein-7 induces activation and transdifferentiation of hepatic stellate cells in vitr[J]o.World J Gastroenterol, 2009, 15(26): 3246-3253.
4	Guo XH, Zhang HY, Zhang QQ, et al.Screening for and validation of a hepatic fibrosis-related pathway induced by insulin-like growth factor-binding protein-related protein 1[J]. Eur J Gastroenterol Hepatol, 2016, 28(7): 762-772.
5	Kawabata K, Sakurai F, Koizumi N, et al.Adenovirus vector mediated gene transfer into stem cells[J]. Mol Pharm, 2006, 3(2): 95-103.
6	McNeish IA, Bell SJ, Lemoine NR.Gene therapy progress and prospects: cancer gene therapy using tumour suppressor genes[J]. Gene Ther, 2004, 11(6): 497-503.
7	Ai YH, Zhang LN, Gong H, et al.Gene transfection efficiency and destination of recombinant adenovirus gene transfer through internal jugular vein injection in rats[J]. Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2005, 30(6): 653-656.
8	Agostini L, Martinon F, Burns K, et al.NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder[J]. Immunity, 2004, 20(3): 319-325.
9	Nikolic-Paterson DJ, Lan HY, Atkins RC.Interleukin-1 receptor antagonism[J]. Semin Nephrol, 1996, 16(6): 583-590.
10	Zeisberg EM, Potenta SE, Suglmoto H, et al.Fibroblasts in kidney fibrosis emerge via endothelial-to-mesenchymal transition[J]. J Am Soe Nephrol, 2008, 19(12): 2282-2287.
11	陈超，武德梅，孙建洁．转化生长因子β与肾脏纤维化的关系[J]．医学综述，2014，20(11)：1961-1962.
12	Begum Q, Noori S, Mahboob T. Antioxidant effect of sodium selenite on thioacetamide-induced renal toxicity[J]. Pakist J Biochem Mol Biol, 2011, 44(1): 21-26.
13	Silva FG.Chemical-induced nephropathy: a review of the renal tubulointerstitial lesions in humans[J]. Toxicol Pathol, 2004, 32(2): 71-84.

组别	n	胶原纤维阳性面积百分比
A组	10	0.18±0.03
B组	25	0.18±0.02
C组(1周)	5	0.19±0.06
C组(2周)	5	0.20±0.09
C组(4周)	5	0.21±0.07
C组(6周)	5	0.23±0.08
C组(9周)	5	0.28±0.10^a

组别	n	胶原纤维阳性面积百分比
A组	10	0.18±0.03
B组	25	0.18±0.02
C组(1周)	5	0.19±0.06
C组(2周)	5	0.20±0.09
C组(4周)	5	0.21±0.07
C组(6周)	5	0.23±0.08
C组(9周)	5	0.28±0.10^a

组别	n	IGFBPrP1	IL-1β	TGFβ1	Fn
A组	10	0.65±0.09	0.19±0.04	0.57±0.07	0.37±0.05
B组	25	0.68±0.08	0.20±0.05	0.58±0.06	0.36±0.04
C组(1周)	5	3.12±0.40^a	0.46±0.05^b	0.91±0.10	0.37±0.04
C组(2周)	5	4.55±0.78^a	0.73±0.08^a	1.98±0.33^a	0.38±0.05
C组(4周)	5	3.15±0.63^a	1.66±0.14^a	2.52±0.42^a	0.39±0.04
C组(6周)	5	1.23±0.08	1.93±0.15^a	3.14±0.59^a	0.41±0.07
C组(9周)	5	0.66±0.06	0.86±0.09^a	3.64±0.71^a	0.60±0.07^a

组别	n	IGFBPrP1	IL-1β	TGFβ1	Fn
A组	10	0.65±0.09	0.19±0.04	0.57±0.07	0.37±0.05
B组	25	0.68±0.08	0.20±0.05	0.58±0.06	0.36±0.04
C组(1周)	5	3.12±0.40^a	0.46±0.05^b	0.91±0.10	0.37±0.04
C组(2周)	5	4.55±0.78^a	0.73±0.08^a	1.98±0.33^a	0.38±0.05
C组(4周)	5	3.15±0.63^a	1.66±0.14^a	2.52±0.42^a	0.39±0.04
C组(6周)	5	1.23±0.08	1.93±0.15^a	3.14±0.59^a	0.41±0.07
C组(9周)	5	0.66±0.06	0.86±0.09^a	3.64±0.71^a	0.60±0.07^a

组别	n	胶原纤维阳性面积百分比
D组	6	0.21±0.05
E组	6	0.29±0.10^a
F组	6	0.24±0.08^bc

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