To study the effects of regulation of nuclear factor-κB(NF-κB)signal by mercaptopyruvate sulfurtransferase(MPST)mediated autophagy on rats with severe acute pancreatitis(SAP).

Fifty SD rats were randomly divided into Sham group, SAP group(severe acute pancreatitis model), SAP+ AAV-NC group(AAV control virus injection+ SAP model), SAP+ AAV-MPST OE group(AAV MPST over-expression virus injection+ SAP model), and SAP+ AAV-MPST shRNA group(AAV MPST knockout virus injection+ SAP model). The severity of pancreatitis using HE staining and pancreatic tissue injury score were evaluated; Autophagic vesicles in pancreatic tissue were observed using electron microscopy; The activity of serum amylase and lipase was detected using colorimetric method; IL-6 levels in pancreatic tissue was detected using ELISA; The expression levels of MPST, LC3 Ⅱ/Ⅰ, beclin1, ATG5, p-NF-κB, NF-κB and β-actin were evaluated by Western blot.

Compared with the SAP group, the pancreatic injury score, number of autophagic vesicles, serum amylase and lipase activity levels, IL-6 level, MPST, LC3 Ⅱ/Ⅰ, beclin1, ATG5, and p-NF-κB/NF-κB levels were significantly increased in SAP+ AAV-MPST OE group, while the SAP+ AAV-MPST shRNA group showed a significant decrease in these indicators(P<0.01).

MPST can induce autophagy and inflammatory response in pancreatic tissue through NF-κB signal, thereby promoting the condition of SAP.Inhibiting MPST is of great significance for the treatment of SAP.

To study the value on clinical application using IDEAL-IQ sequence fat fraction to accurately quantifying abdominal fat volume.

The waistline and weight of 82 patients who underwent IDEAL-IQ sequence examination in Shenzhen Bao′an District Central Hospital from May 2018 to September 2020 were collected retrospectively. The fat fraction obtained from the sequence were processed by AW4.6 post-processing workstation Reformat function. The visceral fat and subcutaneous fat at L2 and L3 vertebral levels were extracted semi-automatically, and the sum of two was the corresponding abdominal fat volume.

The age, weight, waistline of 82 patients was (40.4±10.9) years old, (74.9±9.5)kg, (92.9±8.9)cm. The visceral fat volume at L2 vertebral level was (555.33±177.38)cm³, the volume of subcutaneous fat was (497.59±207.53)cm³, and the volume of abdominal fat was (1052.91±305.81)cm³. The volume of visceral fat at L3 vertebral level was (552.50±184.39)cm³, the volume of subcutaneous fat was (564.01±213.65)cm³, and the volume of abdominal fat was (1086. 51±330.24)cm³. The abdominal fat volume at L2 and L3 level was moderately correlated with weight (r=0.629, 0.671, P<0.01). There was a strong correlation between abdominal fat volume and waistline at L2 and L3 level (r=0.771, 0.805, P<0.01).

IDEAL-IQ sequence equiped with post-processing function, which is fast accurate, noninvasive and safe, provides a semi-automatic technique to accurately quantify body fat content. It is expected to replace traditional CT scanning and become a better options for observation and follow-up for body fat content and distribution.

To determine the efficacy of the endoscopic microradiofrenquency in the treatment of patients with refractory gastroesophageal reflux disease (GERD) using meta-analysis.

Medline, Embase, Cochrane Library, Elsevier, Wanfang, VIP and CNKI were searched by computer to collect literatures on the efficacy of endoscopic microradiofrenquency for refractory GERD. The literatures were screened by inclusion and exclusion criteria, and the data of the selected literatures were extracted. Meta analysis was performed using RevMan 5.3 software.

A total of 9 studies were enrolled, all of which were cohort studies. The meta-analysis results showed that endoscopic microradiofrenquency could reduce the heartburn (mean difference -2.21, P<0.000 01), regurgitation (mean difference -3.85, P<0.000 01), chest pain (mean difference -2.92, P<0.000 01), cough (mean difference -3.87, P<0.000 01) and asthma score (mean difference -4.15, P<0.000 01). The procedure also reduced GERD-health related quality of life (GERD-HRQL) score (mean difference -19.84, P<0.000 01), the use of proton pump inhibitor (PPI) (99.9% vs 41.2%, P<0.000 01) and esophageal inflammation (62.3% vs 34.0%, P<0.000 01). After treatment, the satisfaction score of patients were improved (mean difference 2.46, P<0.000 01), but the difference in the number of satisfied patients was not statistically significant (20.0% vs 87.9%, P=0.28).

Endoscopic microradiofrenquency can significantly relieve patients' heartburn, regurgitation, chest pain, cough, asthma and other symptoms, improve the quality of life, reduce the use of PPI, and relieve esophageal inflammation.

To investigate the clinical presentation and CT/MRI features of malignant pleomorphic adenoma(MPA)of the eye in order to improve the diagnosis of this disease.

The clinical imaging data of 43 patients with MPA in the First Affiliated Hospital of Zhengzhou University from January 2012 to October 2021 were retrospectively analyzed.All patients were monocular disease, including 20 males and 23 females, aged 16-76(53.79±15.54)years old.There were 23 initial cases and 20 recurrent cases, including 13 cases with recurrence once, 3 cases with recurrence twice, 2 cases with recurrence three times, 1 case with recurrence four times, and 1 case with recurrence five times.

The main symptoms and signs of MPA were ocular proptosis, ocular swelling, blurred vision, lacrimation and ocular pain.One case showed pathological manifestation of carcinosarcoma, and 42 cases showed carcinoma ex pleomorphic adenoma.MPA was located in the right eye in 17 cases and left eye in 26 cases; 23 cases originated from the inner myoconus region, 19 cases from the myoconus region, and 1 case from the outer myoconus region; the lesions were irregularly shaped in 23 cases, round or round-like in 14 cases, and shallowly lobulated at the margin in 6 cases.There were 13 cases of clear edge, 15 cases of unclear edge and 15 cases of fuzzy edge.MPA showed limited lesions in 5 cases and diffuse lesions in 38 cases; 28 cases grew deeper along the optic nerve, 11 cases grew outside the orbit, and 4 cases grew laterally, including 1 case invading the anterior skull base, 1 case invading the sinus and 2 cases invading the temporal fossa.CT showed 27 cases of iso-density, 7 cases of slightly low density and 2 cases of slightly high density.Plaque calcification was observed in 7 cases and obvious cystic necrosis was observed in 14 cases.Mild enhancement was observed in 4 cases, moderate enhancement in 5 cases, and uneven enhancement in 8 cases after enhancement.Incomplete capsule was observed in 25 cases.There was no change in adjacent bone in 5 cases, compression deformation in 13 cases and bone destruction/defect in 18 cases.MRI showed slightly low signal in T1WI in 16 cases, iso-signal in 5 cases, and low signal in 4 cases.T2WI showed slightly high signal in 16 cases, high signal in 8 cases, slightly low signal in 1 case; DWI showed slightly high signal in 16 cases, high signal in 5 cases, slightly low signal in 4 cases; signal inhomogeneity in 22 cases; moderate enhancement after enhancement in 2 cases, significant enhancement in 11 cases, and inhomogeneous enhancement in 13 cases; incomplete envelope in 16 cases.

The possibility of MPA should be considered for swelling growth and large masses in the eye, and total tumor resection should be considered without prior incisional biopsy.CT and MRI examinations can accurately visualize the surgical approach of MPA, which is important for the development of treatment plans and prognostic follow-up.

To investigate the effects of dual-source dual-energy computed tomography (CT) scanning with different single-energy reconstructions on the display of interstitial lesions of 2019 novel coronavirus pneumonia (COVID-19), image quality and radiation dose.

The chest plain scan imaging data of 20 patients with COVID-19 from January to March 2020 in the First Affiliated Hospital of Zhengzhou University were retrospectively analyzed. The radiation dose of dual-source dual-energy CT and conventional CT were compared in the same patient, and the single energy images of 40 keV, 60 keV, 80 keV, 100 keV and 120 keV of dual-source dual-energy CT were compared. The CT value and standard deviation (SD) of interstitial lesions with different energy were compared, and signal-noise ratio (SNR) and contrast-noise ratio (CNR) were calculated. The image quality was assessed by two experienced pulmonary diagnostician using the 5-point method, and the differences in image quality among different single-energy groups were compared.

CT dose index (CTDI_vol) in the dual-source dual-energy CT group was (6.0±2.4) mGy, which decreased by 33% compared with the conventional CT group (8.9±2.9) mGy, with a statistically significant difference (t=10.694, P<0.001). dose length product (DLP) and effective dose (ED) in the dual-source dual-energy CT group were (217.5±79.6) mGy·cm and (3.1±1.1) mSv respectively, which were significantly lower than those in the conventional CT group (333.8±109.0) mGy·cm (t=10.727, P<0.001) and (4.7±1.5) mSv (t=10.730, P<0.001). In the reconstructed images of the 5 groups, with the increase of X-ray energy, the SNR and CNR of the single energy group gradually increased, while the CT value and SD value of the images gradually decreased, and there were statistically significant differences between the two adjacent groups (P<0.001). The consistency of the subjective evaluation of the image quality of the two diagnostic physicians was good (Kappa value was between 0.700 and 0.857, P<0.001). There were statistically significant differences in subjective scores of different energy images (P<0.001), and the subjective score of image in the 80 keV group was the highest.

Compared with the conventional CT, the use of dual-source dual-energy CT reduces the radiation dose. The quality score of 120 keV single-energy image is higher, but the 80 keV single-energy image is more conducive to displaying interstitial lesions, which is of great significance for indicating the progression of disease.

To analyze the effect of psychological counseling on emotion and success rate of patients undergoing electronic gastroscopy for the first time.

A total of 174 patients who underwent general electronic gastroscopy for the first time in the Gastrointestinal Endoscopy Center of Suizhou Central Hospital from May 1, 2021 to May 30, 2021 were randomly selected and randomly divided into observation group and control group, including 87 patients in each group.The control group was only given routine nursing care, which included health education and guidance on how to cooperate and body position.On the basis of routine nursing, the observation group was given psychological counseling before, during and after operation.Finally, the emotion, the occurrence of adverse reactions and the success rate of examination were compared between the two groups.

The nervousness and rejection of the patients in the observation group were significantly less than those in the control group, with a statistically significant difference(P<0.05).The degree of adverse reactions in the observation group was significantly less than that in the control group, with a statistically significant difference(P<0.05).The examination success rate of patients in the observation group was 98.85%, which was higher than 90.80% in the control group, with a statistically significant difference(P<0.05).

Psychological counseling for patients undergoing electronic gastroscopy for the first time can significantly relieve the patient′s fear and nervousness, so that the patient can slowly adapt to the examination process, so as to better cooperate with the examination, which can improve the success rate of the patient′s examination.