Abstract:

Objective

To analyze the relationship between the characteristics of intestinal flora and serum zinc, creatine kinase isoenzyme (CK-MB) and inflammatory factors in children with rotavirus infection-related diarrhea.

Methods

A total of 200 children with rotavirus infection-related diarrhea admitted to Handan Maternal and Child Health Hospital from January 2018 to December 2023 were divided into mild group (n=141) and moderate severe group (n=59) according to their condition, and 80 children with non-infectious diarrhea were selected and included in the control group. 16SrRNA fluorescence quantitative polymerase chain reaction (PCR) was used to detect the contents of Bifidobacterium, Escherichia coli and Lactobacillus in feces of children in each group, and the serum zinc,CK-MB and inflammatory factors were detected. The characteristics of intestinal flora in children with rotavirus infection-related diarrhea were analyzed, and Pearson correlation analysis was used to calculate the correlation between fecal intestinal flora content and serum zinc, CK-MB and inflammatory factors.

Results

The contents of Bifidobacterium and Lactobacillus in feces of children in mild group were lower than those in control group, and those in moderate and severe group were lower than those in mild group(P＜0.05). The serum zinc content in mild group was lower than that in control group, and CK-MB was higher than that in control group. The serum zinc content in moderate and severe group was lower than that in mild group, and CK-MB was higher than that in mild group, with statistical significance (P＜0.05). The levels of serum IFN-γ, IL-6, IL-10 and TNF-α in mild group were higher than those in control group, and the levels of serum IFN-γ, IL-6, IL-10 and TNF-α in moderate and severe group were higher than those in mild group (P＜0.05). Pearson correlation analysis showed that the contents of Bifidobacterium and Lactobacillus in children with rotavirus infection-related diarrhea were positively correlated with serum zinc content, and negatively correlated with CK-MB, IFN-γ, IL-6, IL-10 and TNF-α content (P＜0.05).

Conclusion

The main feature of intestinal flora in children with rotavirus infection-related diarrhea is the decrease of Bifidobacterium and Lactobacillus, and the decrease of Bifidobacterium and Lactobacillus is more obvious with the progress of the disease, which is accompanied by the decrease of serum zinc content and the increase of CK-MB and inflammatory factors.

Key words: Rotavirus infection, Diarrhea, Intestinal flora, Trace elements, Creatine kinase isoenzyme, Inflammatory factor