LncRNA GABPB1-AS1 promotes the proliferation, invasion and migration of human colorectal cancer cells by targeting hsa-miR-30b-3p

doi:10.3877/cma.j.issn.2095-2015.2025.05.004

Abstract

Abstract:

Objective

To explore the molecular mechanism by which LncRNA GABPB1-AS1 promotes the proliferation, invasion and migration of human colorectal cancer cells by targeting hsa-miR-30b-3p.

Methods

Human normal colorectal mucosal cell line FHC and human colorectal cancer cell lines HT-29, SW480, LOVO and WiDr were cultured in vitro. HT-29 cells were transfected with si-NC, si-LncRNA GABPB1-AS1, miR-30b-3p inhibitor control and miR-30b-3p inhibitor. The differential expression and potential target binding sites of GABPB1-AS1 and hsa-miR-30b-3p in colorectal cancer tissues and adjacent tissues were predicted through the Starbase database, and the impact of GABPB1-AS1 expression on the survival prognosis of colorectal cancer patients was predicted through the GSCA database. The expression levels of GABPB1-AS1 and miR-30b-3p were detected by RT-qPCR. The targeting relationship between GABPB1-AS1 and hsa-miR-30b-3p was verified by dual-luciferase assay. Cell viability was detected by CCK-8. Cell apoptosis was detected by flow cytometry. Cell invasion ability was detected by Transwell assay. Cell migration ability was detected by scratch assay.

Results

Bioinformatics analysis indicated that compared with normal tissues, GABPB1-AS1 was upregulated in colorectal cancer tissues, while miR-30b-3p was downregulated. Patients with high expression of GABPB1-AS1 had significantly shorter overall survival and progression-free survival. The results of RT-qPCR and dual luciferase experiments showed that GABPB1-AS1 was upregulated in colorectal cancer tissues and miR-30b-3p was downregulated. GABPB1-AS1 negatively regulated the expression of miR-30b-3p. Knockdown of GABPB1-AS1 expression could significantly inhibit the viability, invasion and migration abilities of HT-29 cells, and promote the apoptosis of HT-29 cells. On this basis, inhibition of miR-30b-3p expression could partially reverse the effects of knockdown of GABPB1-AS1 on the viability, apoptosis level, invasion and migration ability of HT-29 cells.

Conclusion

The lncRNA GABPB1-AS1 promotes the proliferation, invasion and migration of human colorectal cancer cells by negatively regulating the expression of hsa-miR-30b-3p.

Key words: Colorectal cancer, HT-29, LncRNA GABPB1-AS1, hsa-miR-30b-3p

Xiaoxiao Tong, Meihua Song, Zheng Fang, Zhengshi Chen. LncRNA GABPB1-AS1 promotes the proliferation, invasion and migration of human colorectal cancer cells by targeting hsa-miR-30b-3p[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2025, 15(05): 436-443.

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References 23

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基因名称	上游引物(5'-3')	下游引物(5'-3')
GABPB1-AS1	GTTCTGCGGTGGGTAGGAGT	CCTCTTGGCTGTCCTTCCGTCTC
miR-30b-3p	TGCGGAGAGGTTGCCCTTGGTGA	TGCGGGTGCTCGCTTCGGCAGC
U6	CTCGCTTCGGCAGCACA	ACGCTTCACGAATTTGCGT

基因名称	上游引物(5'-3')	下游引物(5'-3')
GABPB1-AS1	GTTCTGCGGTGGGTAGGAGT	CCTCTTGGCTGTCCTTCCGTCTC
miR-30b-3p	TGCGGAGAGGTTGCCCTTGGTGA	TGCGGGTGCTCGCTTCGGCAGC
U6	CTCGCTTCGGCAGCACA	ACGCTTCACGAATTTGCGT

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