Association between tumor necrosis factor-α gene polymorphisms and susceptibility to irritable bowel syndrome: a Meta-analysis

doi:10.3877/cma.j.issn.2095-2015.2017.05.005

Abstract

Abstract:

Objective

To investigate the association between tumor necrosis factor-α (TNF-α) gene polymorphisms and genetic susceptibility to irritable bowel syndrome (IBS).

Methods

Publications of case-control studies involving TNF-α gene polymorphisms and susceptibility to IBS from the databases including PubMed, Cochrane Library, EMBase, Chinese National Knowledge Infrastructure (CNKI), Chinese Biological Medical Database (CBM) and Wanfang database were searched up to May 30, 2016. Five genetic models including allele-contrast (G vs. A), dominant (GG vs. AA+ AG), recessive (AA vs. GG+ AG), codominant (GG vs. AG, GG vs. AA) and over-dominant models (AG vs. GG+ AA) were applied to analyze the odds ratio (OR) and 95% confidence interval (CI) of TNF-α 308 (A/G) and 238 (A/G) genetic polymorphism in IBS and control groups. Heterogeneity tests and correlation analyses were performed using Review Manager 5.3.

Results

Seven studies including 1735 IBS patients and 1449 controls were selected in this Meta-analysis. There were seven studies focused on TNF-α 308 polymorphism (four from Europe and Latin America, three from Asia) and two studies on TNF-α 238 polymorphism (both from Europe and Latin America). The whole-analysis results showed that neither alleles A/G nor the genotypes of GG/AG/AA of TNF-α 308/238 were associated with the susceptibility to IBS by five genetic models (P>0.05). Further subgroup analyses by different IBS subtypes showed that neither alleles A/G nor the genotypes of GG/AG/AA of TNF-α 308 were associated with the susceptibility to IBS with diarrhea or constipation. Further subgroup analyses by different populations showed that for TNF-α 308 polymorphism in Asian population (no statistical heterogeneity), GG vs. AA+ AG OR=1.82, 95% CI: 1.08-3.07, P=0.02; AG vs. GG+ AA OR=0.50, 95% CI: 0.29-0.85, P=0.01; GG vs. AG OR=1.97, 95% CI: 1.16-3.37, P=0.01. But TNF-α 308 polymorphism was not significantly associated with the susceptibility to IBS in occidental population.

Conclusions

TNF-α 308 GG and AG genotypes may be associated with the genetic susceptibility to IBS in Asian population. No associations are found between the genetic polymorphisms of TNF-α 308/238 and the genetic susceptibility to IBS in occidental population. The genetic polymorphisms of TNF-α 308 are not associated with the susceptibility to different subtypes of IBS.

Key words: Irritable bowel syndrome, Tumor necrosis factor-α, Genetic polymorphism, Meta-analysis

Hongli Yang, Xiaofei Lei, Kun Li, Changqing Xu, Jing Yang. Association between tumor necrosis factor-α gene polymorphisms and susceptibility to irritable bowel syndrome: a Meta-analysis[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2017, 07(05): 211-219.

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URL: https://zhxhbyyxzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-2015.2017.05.005

https://zhxhbyyxzz.cma-cmc.com.cn/EN/Y2017/V07/I05/211

Figures/Tables 9

References 14

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纳入文献	年份	地区	基因分型方法	IBS诊断标准	病例数	对照数	对照来源
Patrick等	2005	荷兰	PCR-RFLP	RomeⅡ	111	162	健康志愿者
Barkhordari等	2010	伊朗	PCR-SSP	RomeⅢ	67	137	健康志愿者
Lee等	2010	韩国	PCR-RFLP	RomeⅢ	94	88	健康志愿者
Santhosh等	2010	印度	PCR-SSP	RomeⅡ	23	20	健康志愿者
Diego等	2012	墨西哥	PCR-RFLP	RomeⅢ	36	42	临床病例^a
Swan等	2013	英国	Kaspar Assay	RomeⅡ	301	179	健康志愿者
Czogalla等b	2015	英国、美国	Kaspar Assay	RomeⅡ	386	186	健康志愿者

纳入文献	年份	地区	基因分型方法	IBS诊断标准	病例数	对照数	对照来源
Patrick等	2005	荷兰	PCR-RFLP	RomeⅡ	111	162	健康志愿者
Barkhordari等	2010	伊朗	PCR-SSP	RomeⅢ	67	137	健康志愿者
Lee等	2010	韩国	PCR-RFLP	RomeⅢ	94	88	健康志愿者
Santhosh等	2010	印度	PCR-SSP	RomeⅡ	23	20	健康志愿者
Diego等	2012	墨西哥	PCR-RFLP	RomeⅢ	36	42	临床病例^a
Swan等	2013	英国	Kaspar Assay	RomeⅡ	301	179	健康志愿者
Czogalla等b	2015	英国、美国	Kaspar Assay	RomeⅡ	386	186	健康志愿者

纳入文献	AA基因型		AG基因型		GG基因型		等位基因A		等位基因G		HWE^a
纳入文献	病例	对照	病例	对照	病例	对照	病例	对照	病例	对照	HWE^a
Patrick等	1	6	45	42	65	114	47	54	175	270	P＝0.396
Barkhordari等	1	0	11	39	55	98	13	39	121	235	P＝0.052
Lee等	0	0	11	15	83	73	11	15	177	161	P＝0.382
Santhosh等	1	0	1	5	21	15	3	5	43	35	P＝0.523
Diego等	0	0	2	3	34	39	2	3	70	81	P＝0.810
Swan等	/	/	/	/	/	/	57	34	244	145	P>0.05^b
Czogalla等	18	5	110	52	258	129	146	62	626	310	P＝0.930

纳入文献	AA基因型		AG基因型		GG基因型		等位基因A		等位基因G		HWE^a
纳入文献	病例	对照	病例	对照	病例	对照	病例	对照	病例	对照	HWE^a
Patrick等	1	6	45	42	65	114	47	54	175	270	P＝0.396
Barkhordari等	1	0	11	39	55	98	13	39	121	235	P＝0.052
Lee等	0	0	11	15	83	73	11	15	177	161	P＝0.382
Santhosh等	1	0	1	5	21	15	3	5	43	35	P＝0.523
Diego等	0	0	2	3	34	39	2	3	70	81	P＝0.810
Swan等	/	/	/	/	/	/	57	34	244	145	P>0.05^b
Czogalla等	18	5	110	52	258	129	146	62	626	310	P＝0.930

纳入文献	AA基因型		AG基因型		GG基因型		等位基因A		等位基因G		HWE^a
纳入文献	病例	对照	病例	对照	病例	对照	病例	对照	病例	对照	HWE^a
Diego等	0	0	2	3	34	39	2	3	70	81	P＝0.810
Czogalla等	2	0	44	22	340	164	48	22	724	350	P＝0.391

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