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中华消化病与影像杂志(电子版)

中华消化病与影像杂志(电子版) ›› 2020, Vol. 10 ›› Issue (06) : 248 -251. doi: 10.3877/cma.j.issn.2095-2015.2020.06.003

所属专题： 总编推荐； 文献；

临床研究

克罗恩病患者血浆中鸟苷与尿鸟苷水平的临床研究
徐东燕1, 张晓雨2, 赵海剑2, 王健2,†(), 孙静3   
  1. 1. 223002　江苏省，淮安市第二人民医院消化内科
    2. 223002　江苏省，淮安市第二人民医院胃肠外科
    3. 214000　江苏省，南京医科大学附属无锡市人民医院消化内科
  • 收稿日期:2020-06-05 出版日期:2020-12-01
  • 通信作者: 王健
  • 基金资助:
    国家自然科学基金(81800490); 江苏省"333"高层次人才项目(BRA2017247); 江苏省六大人才高峰项目(WSW-220)

Clinical study on plasma levels of guanylin and uroguanylin in patients with Crohn disease

Dongyan Xu1, Xiaoyu Zhang2, Haijian Zhao2, Jian Wang2,†(), Jing Sun3   

  1. 1. Department of Gastroenterology, Second People′s Hospital of Huai′an, Jiangsu Province, Huai′an 223002, China
    2. Department of Gastrointestinal Surgery, Second People′s Hospital of Huai′an, Jiangsu Province, Huai′an 223002, China
    3. Department of Gastroenterology, Wuxi People′s Hospital Affiliated to Nanjing Medical University, Wuxi 214000, China
  • Received:2020-06-05 Published:2020-12-01
  • Corresponding author: Jian Wang
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徐东燕, 张晓雨, 赵海剑, 王健, 孙静. 克罗恩病患者血浆中鸟苷与尿鸟苷水平的临床研究[J]. 中华消化病与影像杂志(电子版), 2020, 10(06): 248-251.

Dongyan Xu, Xiaoyu Zhang, Haijian Zhao, Jian Wang, Jing Sun. Clinical study on plasma levels of guanylin and uroguanylin in patients with Crohn disease[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2020, 10(06): 248-251.

目的

评价克罗恩病(CD)患者血浆鸟苷前体激素(ProGN)和尿鸟苷前体激素(ProUGN)水平在临床中的价值。

方法

采用酶联免疫吸附试验(ELISA)对克罗恩病患者和健康对照者血浆ProGN和ProUGN水平进行检测，并分析其与临床疾病活动性及24 h大便次数的相关性。

结果

与健康对照者相比，CD患者血浆ProGN与ProUGN水平均明显降低(均P<0.05)。亚组分析显示，缓解期与活动期患者血浆ProGN水平均显著低于健康对照组(均P<0.001)，活动期患者水平低于缓解期患者，但差异无统计学意义(P＝0.121)；缓解期患者血浆ProUGN水平显著低于健康对照组(P<0.001)，活动期患者低于健康对照组，但差异无统计学意义(P＝0.266)；缓解期患者显著低于活动期患者，差异有统计学意义(P＝0.011)。Pearson相关性分析显示，血浆ProGN及ProUGN水平与患者24 h大便次数均呈显著负相关(r＝-0.804与r＝-0.767，均P<0.001)。血浆ProGN水平与Best克罗恩病活动指数呈显著负相关(r＝-0.561，P<0.001)；而ProUGN水平与Best克罗恩病活动指数无线性相关(r＝-0.155，P＝0.314)。

结论

克罗恩病患者疾病临床活动性和腹泻可能是血浆ProGN水平的决定因素。

关键词: 克罗恩病, 鸟苷, 尿鸟苷
Objective

To evaluate the clinical value of ProGN and ProUGN in patients with Crohn disease(CD).

Methods

The plasma ProGN and ProUGN levels of Crohn disease patients and healthy controls were detected by enzyme-linked immunosorbent assay(ELISA), and the correlation between plasma levels and clinical disease activity and 24-hour stool frequency was analyzed.

Results

Compared with the healthy controls, the plasma ProGN and ProUGN levels of CD patients were significantly lower(P<0.05). Subgroup analysis showed that the plasma ProGN level of patients in remission period and active period was significantly lower than that of healthy control group(P<0.001), while the level of patients in active period was lower than that of patients in remission period, but there was no statistical significance(P=0.121); the plasma ProUGN level of patients in remission period was significantly lower than that in healthy control group(P<0.001), and plasma level of patients in active period was lower than that in healthy control group, but there was no statistical significance(P=0.266), and plasma level of patients in remission period was significantly lower than that in active period(P=0.011). Pearson correlation analysis showed that plasma ProGN and ProUGN levels were negatively correlated with 24-hour stool frequency(r=-0.804, P<0.001; r=-0.767, P<0.001). There was a significant negative correlation between the plasma ProGN level and the Best Crohn disease activity index(r=-0.561, P<0.001), but there was no linear correlation between the plasma ProGN level and the Best Crohn disease activity index(r=-0.155, P=0.314).

Conclusion

The clinical activity and diarrhea may be the determinants for level of proGN in plasma.

Key words: Crohn disease, Guanylin, Uroguanylin
表1 克罗恩病患者与健康对照组血浆ProGN与ProUGN水平的比较(ng/ml)
项目 ProGN ProUGN
克罗恩病患者(n＝44) 5.24±0.39 2.26±0.42
健康对照组(n＝20) 7.68±0.36 2.62±0.41
t值 -23.69 -3.194
P值 <0.001 0.002
图1 克罗恩病患者鸟苷前体激素(ProGN)和尿鸟苷前体激素(ProUGN)血浆水平亚组分析
图2 患者24 h大便次数与患者血浆鸟苷前体激素(ProGN)和尿鸟苷前体激素(ProUGN)水平相关性分析
图3 患者血浆鸟苷前体激素(ProGN)水平与疾病活动指数相关性分析
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