Preliminary experimental study on the expression of α1 adrenergic receptor subtypes in human liver sinusoidal endothelial cells

doi:10.3877/cma.j.issn.2095-2015.2022.02.007

Abstract

Abstract:

Objective

To investigate the expressions of α₁ adrenergic receptor (α₁AR) subtypes in liver sinusoidal endothelial cells (LSEC).

Methods

Human primary LSEC were cultured in vitro. The effects of α₁AR agonists phenylephrine and α₁AR blocker doxazosin on the expressions of angiogenesis-related markers CD31 and vWF in LSEC and the expressions of various α₁AR subtypes in LSEC were verified by Western blotting analysis. Immunohistochemistry was used to verify the expressions of α₁AR subtypes in mouse liver.

Results

α₁AR agonist phenylephrine could increase the expressions of CD31 and vWF in LSEC, and α₁AR blocker doxazosin antagonized this effect. The expressions of α_1aAR and α_1bAR were detected in LSEC in vivo and in vitro, but the expression of α_1dAR was not detected.

Conclusions

α₁AR is involved in the regulation of LSEC phenotype, and this function is achieved through two subtypes of α_1aAR and α_1bAR. Therefore, α₁AR may become a new regulatory target of liver fibrosis.

Key words: Liver fibrosis, α₁ adrenergic receptor, Liver sinusoidal endothelial cells

Qian Ding, Zhen Li, Sining Wang, Lianhui Zhao, Guangchuan Wang, Chunqing Zhang. Preliminary experimental study on the expression of α₁ adrenergic receptor subtypes in human liver sinusoidal endothelial cells[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2022, 12(02): 98-101.

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URL: https://zhxhbyyxzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-2015.2022.02.007

https://zhxhbyyxzz.cma-cmc.com.cn/EN/Y2022/V12/I02/98

Figures/Tables 3

References 21

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组别	CD31/Tubulin相对灰度值	vWF/Tubulin相对灰度值
对照组	1	1
去氧肾上腺素组	1.614±0.202^a	1.246±0.150^a
去氧肾上腺素+多沙唑嗪组	0.969±0.153^b	0.761±0.151^b

组别	CD31/Tubulin相对灰度值	vWF/Tubulin相对灰度值
对照组	1	1
去氧肾上腺素组	1.614±0.202^a	1.246±0.150^a
去氧肾上腺素+多沙唑嗪组	0.969±0.153^b	0.761±0.151^b

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