Effect of compound Lactobacillus acidophilus tablets combined with montmorillonite powder on diarrhea symptoms and intestinal microbiota in children with rotavirus enteritis

doi:10.3877/cma.j.issn.2095-2015.2025.03.017

Abstract

Abstract:

Objective

To explore the effects of compound Lactobacillus acidophilus tablets combined with montmorillonite powder on diarrhea symptoms and intestinal microbiota in children with rotavirus enteritis.

Methods

A total of 80 children with rotavirus enteritis admitted to Jieshou People's Hospital from January 2022 to February 2024 were selected, and divided into two groups according to the random number table method.The control group (40 cases) received treatment with montmorillonite powder, while the observation group (40 cases) received treatment with compound Lactobacillus acidophilus tablets combined with montmorillonite powder.After treatment, the efficacy, clinical symptom relief time (fever, diarrhea, antiemesis, dehydration, stool characteristics), gut microbiota (Bifidobacterium,Lactobacillus, Escherichia coli), and immune function (CD4⁺, CD8⁺, CD3⁺, CD4⁺/CD8⁺), inflammatory response [C-reactive protein (CRP), tumor necrosis factor alpha (TNFα), interleukin (IL-1β, IL-18)] were compared between the two groups.

Results

The total clinical effective rate of the observation group was 95.00%, which was higher than that of the control group 77.50% (χ²=5.441, P<0.05).The observation group had shorter fever, diarrhea, dehydration, stool characteristics relief time, and antiemetic time compared to the control group (t=3.038, 3.167, 4.284, 2.738, 3.241, P<0.05).After 3 days of treatment, the number of Lactobacilli and Bifidobacteria in the observation group increased compared to the control group, while the number of Escherichia coli decreased (t=2.164, 2.843, 3.016, P<0.05).CD3⁺, CD4⁺, and CD4⁺/CD8⁺ in the observation group were all higher than those in the control group, and CD8⁺ was lower than that in the control group (t=3.152, 4.274, 3.975, 4.628, P<0.05).After 3 days of treatment, the levels of serum CRP, IL-1β, TNFα, and IL-18 in the observation group were lower than those in the control group(t=5.729, 5.823, 10.962, 5.317, P<0.05).

Conclusion

The combination of compound Lactobacillus acidophilus tablets and montmorillonite powder significantly improves clinical symptoms such as diarrhea and fever in rotavirus enteritis, and restores the balance of intestinal microbiota.

Key words: Rotavirus enteritis, Compound Lactobacillus acidophilus tablets, Montmorillonite scattering, Diarrhea, Intestinal microbiota

Qian Wang, Dengfeng Li, Yongchun Tian, Xiuling Li, Rui Wang. Effect of compound Lactobacillus acidophilus tablets combined with montmorillonite powder on diarrhea symptoms and intestinal microbiota in children with rotavirus enteritis[J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2025, 15(03): 277-281.

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URL: https://zhxhbyyxzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-2015.2025.03.017

https://zhxhbyyxzz.cma-cmc.com.cn/EN/Y2025/V15/I03/277

Figures/Tables 6

组别	例数	性别[例(%)]		年龄(岁, $\overset{ˉ}{x}$ ± s )	病程(d, $\overset{ˉ}{x}$ ± s )	体重(kg, $\overset{ˉ}{x}$ ± s )	腹泻次数(次/d, $\overset{ˉ}{x}$ ± s )	脱水程度[例(%)]
组别	例数	男	女	年龄(岁, $\overset{ˉ}{x}$ ± s )	病程(d, $\overset{ˉ}{x}$ ± s )	体重(kg, $\overset{ˉ}{x}$ ± s )	腹泻次数(次/d, $\overset{ˉ}{x}$ ± s )	轻度	中度	重度
对照组	40	23(57.50)	17(42.50)	1.89±0.58	1.53±0.52	11.78±2.72	5.16±1.09	25(62.50)	10(25.00)	5(12.50)
观察组	40	24(60.00)	16(40.00)	1.91±0.56	1.51±0.54	11.63±2.69	5.13±1.10	24(60.00)	10(25.00)	6(15.00)
χ ²/t值		0.531		0.732	0.372	0.306	0.472		0.834
P值		0.486		0.316	0.717	0.625	0.517		0.210

组别	例数	性别[例(%)]		年龄(岁, $\overset{ˉ}{x}$ ± s )	病程(d, $\overset{ˉ}{x}$ ± s )	体重(kg, $\overset{ˉ}{x}$ ± s )	腹泻次数(次/d, $\overset{ˉ}{x}$ ± s )	脱水程度[例(%)]
组别	例数	男	女	年龄(岁, $\overset{ˉ}{x}$ ± s )	病程(d, $\overset{ˉ}{x}$ ± s )	体重(kg, $\overset{ˉ}{x}$ ± s )	腹泻次数(次/d, $\overset{ˉ}{x}$ ± s )	轻度	中度	重度
对照组	40	23(57.50)	17(42.50)	1.89±0.58	1.53±0.52	11.78±2.72	5.16±1.09	25(62.50)	10(25.00)	5(12.50)
观察组	40	24(60.00)	16(40.00)	1.91±0.56	1.51±0.54	11.63±2.69	5.13±1.10	24(60.00)	10(25.00)	6(15.00)
χ ²/t值		0.531		0.732	0.372	0.306	0.472		0.834
P值		0.486		0.316	0.717	0.625	0.517		0.210

组别	例数	显效	有效	无效	有效率
对照组	40	12(30.00)	19(47.50)	9(22.50)	31(77.50)
观察组	40	21(52.50)	17(42.50)	2(5.00)	38(95.00)

组别	例数	发热缓解时间	腹泻缓解时间	止吐时间	脱水缓解时间	大便性状缓解时间
对照组	40	1.34±0.53	2.88±0.74	2.27±0.52	2.07±0.52	2.03±0.54
观察组	40	0.92±0.49	1.27±0.47	1.42±0.55	1.35±0.51	1.01±0.52
t值		3.032	3.712	3.241	4.084	2.239
P值		0.021	0.017	0.015	0.010	0.022

组别	例数	时间点	乳酸杆菌(logCFU/g)	双歧杆菌(logCFU/g)	大肠杆菌(logCFU/g)
对照组	40	治疗前	6.81±0.30	7.48±0.65	8.24±0.42
		治疗3 d 后	7.27±0.42^a	8.11±0.68^a	7.63±0.35^a
观察组	40	治疗前	6.85±0.35	7.43±0.66	8.21±0.49
		治疗3 d 后	7.85±0.67^ab	8.93±0.77^ab	6.14±0.21^ab

组别	例数	时间点	CD3⁺(%)	CD4⁺(%)	CD8⁺(%)	CD4⁺/CD8⁺
对照组	40	治疗前	44.04±4.07	28.16±2.43	39.28±3.74	0.76±0.14
		治疗3 d 后	47.76±4.27^a	31.37±2.52^a	33.27±2.81^a	0.92±0.17^a
观察组	40	治疗前	44.97±4.01	28.22±2.39	39.69±3.81	0.75±0.15
		治疗3 d 后	50.73±4.59^ab	37.45±3.15^ab	28.37±2.95^ab	1.21±0.18^ab

组别	例数	时间点	IL-1β(ng/ml)	TNF-α(μg/L)	IL-18(μg/L)	CRP(mg/L)
对照组	40	治疗前	64.18±7.68	61.46±7.51	194.32±29.15	32.62±4.71
		治疗3 d 后	36.29±4.66^a	45.38±6.11^a	165.17±26.11^a	13.23±3.82^a
观察组	40	治疗前	64.45±7.26	61.45±7.50	194.44±29.14	32.85±4.62
		治疗3 d 后	33.23±4.48^ab	37.21±4.12^ab	106.27±24.86^ab	9.78±3.56^ab

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